Rapid enumeration of microorganisms in foods by the …
Rapid enumeration of microorganisms in foods by the direct epifluorescent filter technique (DEFT) N. Jaeggi 1 Experientia volume 42, page 95 ( 1986 ) Cite this article
direct epifluorescent filter technique deft
direct epifluorescent filter technique deft
Contact us +The detection of irradiated foods using the Direct ...
The technique will give an indication of the total number of viable micro-organisms present before irradiation. It is based on the comparison of an aerobic plate count () with a count obtained using the Direct Epifluorescent Filter Technique (DEFT).
Direct epifluorescent filter technique (DEFT). Use in ...
In 1984-88, 792 samples of drinking water were studied using DEFT and results were compared with those of plate counts on King''s B agar. The 2 tests required 20 min and 72 h respectively. DEFT total counts were up to 5000 times higher than plate counts, suggesting that drinking water contains many... Direct epifluorescent filter technique (DEFT).
Collaborative trial of the direct epifluorescent filter ...
The direct epifluorescent filter technique (DEFT) is a new rapid method which uses membrane filtration and epifluorescent microscopy for counting in milk. A collaborative trial of the DEFT was conducted between six laboratories. Each laboratory obtained a highly significant relationship between the DEFT count and plate count with a correlation ...
The direct epifluorescent filter technique (DEFT ...
With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram-staining procedure using acridine orange as the counterstain. The method enumerated viable Gram-negative and all Gram-positive bacteria.
A modified direct epifluorescent filter technique for the ...
1990-12-1 · A modified direct epifluorescent filter technique (DEFT) for the detection and enumeration of visible yeast in fruit‐flavoured yoghurt is described. The method involves an initial enrichment in oxytetracycline glucose yeast peptone broth (OGYP, 30d̀C/24, 48 h), prefiltration prior to DEFT and use of the vital stain Viablue 1. Additional yoghurt samples were subjected to prolonged incubation ...
Comparison of antibody-direct epifluorescent filter ...
The antibody-direct epifluorescent filter (Ab-DEFT) technique was evaluated as a rapid alternative to the most probable number (MPN) method for enumeration of artificially inoculated Listeria monocytogenes in ready-to-eat packaged salads and other fresh vegetables. Ab-DEFT was performed by homogenization of food in mesh-lined Stomacher bags ...
(PDF) The Direct Epifluorescent Filter Technique for the ...
Two to ten milliliters of these prefiltered suspensions could be filtered in the direct epifluorescent filter technique (DEFT). The technique takes less than 30 min to complete and has a lower ...
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2015-10-19 · 2.1.2 (direct epifluorescent filter technique, DEFT) TritonX–100 , 。
The direct epifluorescent filter technique, cytochrome c ...
1986-4-1 · The ability of the direct epifluorescent filter technique (DEFT) to predict the keeping quality (KQ) of pasteurized cream, after an 18-h preincubation stage, was poor unless (particularly with samples that had a corresponding long KQ) undiluted cream was filtered. The DEFT then gave as good, if not better, prediction of the KQ as the plate count.
Review: The direct epifluorescent filter technique ...
The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the method are discussed.
DEFT
DEFT stands for Direct Epifluorescent Filter Techniques. DEFT is defined as Direct Epifluorescent Filter Techniques rarely. DEFT stands for Direct Epifluorescent Filter Techniques. Printer friendly. Menu Search "AcronymAttic . Abbreviation to define. Find. Examples: NFL, NASA, PSP, HIPAA.
The direct epifluorescent filter technique (DEFT ...
1995-1-1 · The direct epifluorescent filter technique (DEFT): increased selectivity, sensitivity and rapidity. Rodrigues UM, Kroll RG. J Appl Bacteriol, 59(6):493-499, 01 …
The Direct Epifluorescent Filter Technique (DEFT ...
Holah, J. T., Betts, R. P., and Thorpe, R. H. (1988) The use of direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) to assess microbial populations on food contact surfaces. J. Appl. Bacteriol. 65, 215–222. PubMed Google Scholar
The use of direct epifluorescent microscopy (DEM) and the ...
Two rapid methods, direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) on swab resuspension fluids, were compared with the traditional total viable count (TVC) on swab resuspension fluids for their ability to enumerate surface populations of attached bacteria.
Application of the direct epifluorescent filter technique ...
1985-7-1 · The direct epifluorescent filter technique (DEFT) and the standard plate count (SPC) were compared in the examination of raw and heat-treated meat products. The most promising results were obtained with raw ground beef, where good agreement was found between DEFT and the SPC.
Status and Future of the Direct Epifluorescent Filter ...
The direct epifluorescent filter technique (DEFT) was originally developed for counting bacteria in raw milk at the National Institute for Research in Dairying, Reading (Pettipher et al. 1980; Pettipher 1983). The method, which uses membrane filtration and epifluorescence microscopy, takes less than 30 min to complete and does not suffer from ...
Fluorescent Antibodies Applied to Direct Epifluorescent ...
In a modification of the direct epifluorescent filter technique (DEFT), direct fluorescent antibody staining was used for the rapid (<1 h), specific enumeration of foodbome Escherichia coli 0157:H7 by epifluorescence microscopy. Cell counts obtained by this method (Ab-DEFT) correlated well with D …
Review: The direct epifluorescent filter technique ...
The direct epifluorescent filter technique (DEFT) for the rapid enumeration of microorganisms is described with particular reference to milk and foods. Recent development with regard to applications and automation, and the likely future of the method are discussed. Citing …
Direct Epifluorescent Filter Technique Deft
AbstractThe direct epifluorescent filter technique DEFT ., The direct epifluorescent filter technique DEFT is a new rapid method which usesmembrane filtration and epifluorescent microscopy for counting in milk. A collaborativetrial of the DEFT was conducted between six laboratories.
Collaborative trial of the direct epifluorescent filter ...
The direct epifluorescent filter technique (DEFT) is a new rapid method which uses membrane filtration and epifluorescent microscopy for counting bacteria in milk. A collaborative trial of the DEFT was conducted between six laboratories. Each laboratory obtained a highly significant relationship between the DEFT count and plate count with a ...
Use of the direct epifluorescent filter technique for the ...
The ability of the direct epifluorescent filter technique (DEFT) to enumerate the viable numbers of various species of yeasts was evaluated. A DEFT count could be made in less than 10 min and the DEFT counts of non-heat-treated samples agreed well with plate counts. The DEFT was unsuitable for the enumeration of yeasts in heat-treated samples ...
Use of the direct epifluorescent filter technique for the ...
The ability of the direct epifluorescent filter technique (DEFT) to enumerate the viable numbers of various species of yeasts was evaluated. A DEFT count could be made in less than 10 min and the DEFT counts of non-heat-treated samples agreed well with plate counts. The DEFT was unsuitable for the e …
Semi-automated direct epifluorescent filter technique for ...
The Direct Epifluorescent Filter Technique (DEFT) and the reference method of counting total bacterial colonies on Petri dishes were compared. IDF Standards 128 (1985) and 161A (1995) were applied. A total of 496 samples of milk were analyzed. Colony forming units per microL milk were transformed to decimal logarithmic units: log (cfu/microL).
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EN 13783-2001,This European Standard specifies a microbiological screening method for the detection of irradiation treatment ofherbs and spices, using the combined direct epifluorescent filter technique (DEFT) and aerobic plate count ().The DEFTIAPC ...
Direct Epifluorescent Filtration Technique DEFT
2019-7-13 · Direct Epifluorescent Filtration Technique DEFT. Last Updated on Sat, 13 Jul 2019 | Media Fills. This technique has also been extensively used in the food industry. It utilizes the observation that viable cells, when exposed to acridine orange, can be visualized under a fluorescent microscope, appearing bright orange, while nonviable cells ...
Use of the Direct Epifluorescent Filter Technique for the ...
J. T. Holah, R. P. Betts, and R. H. Thorpe (1988) The use of direct epifluorescent microscopy (DEM) and the direct epifluorescent filter technique (DEFT) to asses microbial population on food contact surfaces. J. Appl. Bacteriol. 65, 215–221. Google Scholar 10.
[PDF] Antibody-direct epifluorescent filter technique for ...
Artificially inoculated Escherichia coli O157:H7 was directly enumerated in ground beef and beef exudate, without enrichment or selection, by the antibody-direct epifluorescent filter technique (Ab-DEFT). The total assay time of the Ab-DEFT was less than 1 h. The beef was homogenized, treated for 15 min with trypsin and Triton X-100, and passed through a 5-microns-pore-size prefilter and then ...
The direct epifluorescent filter technique (DEFT): increased sel…
With the direct epifluorescent filter technique (DEFT), differentiation of bacteria was achieved by a modified Gram-staining procedure using acridine orange as the counterstain. The method enumerated viable Gram-negative and all Gram-positive bacteria. Counts of clumps of orange fluorescent cells (G …
Use of the direct epifluorescent filter technique for the ...
The ability of the direct epifluorescent filter technique (DEFT) to enumerate the viable numbers of various species of yeasts was evaluated. A DEFT count could be made in less than 10 min and the DEFT counts of non-heat-treated samples agreed well with plate counts.